Read e-book online A Novel Heme-Thiolate Peroxygenase AaeAPO and Its PDF

By Xiaoshi Wang

during this thesis, Xiaoshi Wang investigates the functionality and mechanism of a newly came upon heme-thiolate peroxygenase, AaeAPO. This enzyme type comes from Agrocybe aegerita and is utilized in the conversion of inert hydrocarbons to alcohols. Xiaoshi's paintings specializes in an extracellular P450 enzyme which isn't constrained in its balance and shortage of solubility and accordingly is suitable for common commercial use. the writer demonstrates that the peroxygenase catalyzes a variety of reactions. occasionally the writer even describes very tricky differences in molecules which are hugely inert. Her particular investigations supply a mechanistic framework for a way the peroxygenase catalyzes the sort of huge variety of reactions. an important spotlight of this thesis is the id of key short-lived intermediates within the catalytic cycle of the peroxygenase, utilizing fast kinetic and spectroscopic tools, in addition to the elucidation of the thermodynamic homes of those high-energy intermediates. This paintings provides new perception into an enormous classification of enzymes.

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A Novel Heme-Thiolate Peroxygenase AaeAPO and Its Implications for C-H Activation Chemistry (Springer Theses) by Xiaoshi Wang

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